| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; DIP-1; Mind bomb homolog 1; RING-type E3 ubiquitin transferase MIB1 ; Zinc finger ZZ type with ankyrin repeat domain protein 2; MIB1; DIP1; KIAA1323; ZZANK2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MIB1 (Arylamine N-acetyltransferase 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Mib1/Mindbomb Antibody Picoband® catalog # A01387-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732). (reported region: H465-E732).
- Molecular weight context: reported MW: 110 kDa; calculated MW: 33899 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Arylamine N-acetyltransferase 1; mindbomb E3 ubiquitin protein ligase 1. E3 ubiquitin-protein ligase MIB1 is an enzyme that in humans is encoded by the MIB1 gene. It is mapped to 18q11.2. This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). Functional note: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. Reported localization: Cytoplasm. Expression/tissue context: Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Research relevance and current trends
- Signaling Pathways: Researchers commonly examine how MIB1 (Arylamine N-acetyltransferase 1) relates to this theme using model systems and orthogonal readouts.
- Stem Cells: Researchers commonly examine how MIB1 (Arylamine N-acetyltransferase 1) relates to this theme using model systems and orthogonal readouts.
- Surface Molecules: Researchers commonly examine how MIB1 (Arylamine N-acetyltransferase 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MIB1 (Arylamine N-acetyltransferase 1) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
