| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial dynamics protein MIEF1; Mitochondrial dynamics protein of 51 kDa; Mitochondrial elongation factor 1; Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like; SMCR7-like protein; MIEF1; MID51; SMCR7L |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MIEF1 recombinant protein (Position: S189-T463). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitochondrial dynamics protein MIEF1 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry, immunoprecipitation and ELISA. The predicted molecular weight is 51.3 kDa, and the supplier reports an observed band at about 51 kDa.
MIEF1 (also called MiD51) is a mitochondrial outer membrane protein that recruits the fission GTPase DRP1 (DNM1L) to the mitochondrial surface. It binds ADP, which it needs to stimulate DRP1 assembly and GTPase activity, and it is studied for its role in balancing mitochondrial fission and fusion.
| Target | Mitochondrial dynamics protein MIEF1 (gene MIEF1; UniProt Q9NQG6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 51.3 kDa |
| Observed MW | 51 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HT1080 cells, human Jurkat cells, human K562 cells, human HL-60 cells, human HEK293 cells, human U-937 cells, human HepG2 cells, rat heart tissue, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human renal clear cells carcinoma tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Samples with a confirmed result (IP): K562 cells.
Immunogen
Recombinant human MIEF1 fragment (Ser189–Thr463), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial dynamics protein MIEF1 in the mitochondrion outer membrane. Tissue expression noted by UniProt (human): Expression is relatively high in heart, skeletal muscle, pancreas and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yanqi Lin et al. (2023) miR-494-5p mediates the antioxidant activity of EPA by targeting the mitochondrial elongation factor 1 gene MIEF1 in HepG2 cells. Journal of Nutritional Biochemistry. 10.1016/j.jnutbio.2023.109279
