| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA;1.14.11.-;60S ribosomal protein L27a histidine hydroxylase;Histone lysine demethylase MINA;MYC-induced nuclear antigen;Mineral dust-induced gene protein;Nucleolar protein 52;Ribosomal oxygenase MINA;ROX;MINA ;MDIG , MINA53 , NO52 ; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MINA53 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAAO-13 is a rabbit monoclonal antibody that recognizes ribosomal oxygenase 2 (gene RIOX2) in human and mouse samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 52.8 kDa, and the supplier reports an observed band at about 53 kDa.
RIOX2 (also called MINA53) is a JmjC-domain oxygenase that hydroxylates a histidine residue in ribosomal protein RPL27A and can also demethylate histone H3 at lysine 9. Originally identified as a MYC-induced nuclear antigen, it is studied in cell growth, ribosome biology and cancer.
| Target | Ribosomal oxygenase 2 (gene RIOX2; UniProt Q8IUF8, human) |
|---|---|
| Clone | AAAO-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.8 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human MINA53.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places ribosomal oxygenase 2 in the nucleus. Tissue expression noted by UniProt (human): Expressed in liver, skeletal muscle, heart, pancreas, and placenta.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.