Anti-MINA53 Rabbit Monoclonal Antibody

SKU:BHA21008313
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Boster Bio
Boster Bio
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Overview
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Anti-MINA antibody (Rabbit monoclonal, clone AAAO-13, Rabbit IgG). Validated for Western blot and immunofluorescence. Reactivity: Human, Mouse. Recognizes ribosomal oxygenase 2. Observed band in Western blot: about 53 kDa.
Target MINA
clone number AAAO-13
Host Rabbit
Reactivity Human,Mouse
Isotype Rabbit IgG
Application(s) WB, ICC, IF
Options selector
Catalog no. Size Conjugation
M00783 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,ICC,IF
      Application details: WB 1:500-2000<br>ICC/IF 1:50-200<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MINA
Alternative names Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA;1.14.11.-;60S ribosomal protein L27a histidine hydroxylase;Histone lysine demethylase MINA;MYC-induced nuclear antigen;Mineral dust-induced gene protein;Nucleolar protein 52;Ribosomal oxygenase MINA;ROX;MINA ;MDIG , MINA53 , NO52 ;
Gene ID 84864
UniProt # Q8IUF8
Host Rabbit
Clone AAAO-13
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
  • Immunofluorescence
Immunogen A synthesized peptide derived from human MINA53
Molecular weight 52.8 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus . Nucleus, nucleolus .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00783
Main SKU BHA21008313
Rabbit Monoclonal

Product Overview

Clone AAAO-13 is a rabbit monoclonal antibody that recognizes ribosomal oxygenase 2 (gene RIOX2) in human and mouse samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 52.8 kDa, and the supplier reports an observed band at about 53 kDa.

RIOX2 (also called MINA53) is a JmjC-domain oxygenase that hydroxylates a histidine residue in ribosomal protein RPL27A and can also demethylate histone H3 at lysine 9. Originally identified as a MYC-induced nuclear antigen, it is studied in cell growth, ribosome biology and cancer.

Target Ribosomal oxygenase 2 (gene RIOX2; UniProt Q8IUF8, human)
Clone AAAO-13
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 52.8 kDa
Observed MW 53 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200

Immunogen

Synthetic peptide based on the sequence of human MINA53.

Reactivity Notes

The supplier lists reactivity with human and mouse. UniProt places ribosomal oxygenase 2 in the nucleus. Tissue expression noted by UniProt (human): Expressed in liver, skeletal muscle, heart, pancreas, and placenta.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 52.8 kDa; observed band at about 53 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human MINA53.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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