| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inositol oxygenase; Aldehyde reductase-like 6; Kidney-specific protein 32; Myo-inositol oxygenase; MI oxygenase; Renal-specific oxidoreductase; MIOX; ALDRL6; KSP32; RSOR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MIOX recombinant protein (Position: M1-K266). Human MIOX shares 89.8% |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects inositol oxygenase (gene MIOX) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
Myo-inositol oxygenase (MIOX) is a non-heme iron enzyme that oxidizes myo-inositol to D-glucuronic acid, the first step of the main pathway for inositol breakdown. It is expressed mainly in the kidney proximal tubule and is studied in inositol metabolism and kidney injury.
| Target | Inositol oxygenase (gene MIOX; UniProt Q9UGB7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human MIOX fragment (Met1–Lys266), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places inositol oxygenase in the cytoplasm. Tissue expression noted by UniProt (human): Kidney specific.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
