| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32;MITF;BHLHE32; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MiTF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOED-13 is a rabbit monoclonal antibody that recognizes microphthalmia-associated transcription factor (gene MITF) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 58.8 kDa, and the supplier reports an observed band at about 52 kDa.
MITF is a basic helix-loop-helix leucine zipper transcription factor that is the master regulator of melanocyte development, survival and pigmentation. It activates pigment genes such as tyrosinase through E-box and M-box elements and is controlled by mTOR in response to nutrients, so it is widely studied in melanocyte biology, melanoma and lysosome regulation. A related Mitf protein is also found in Drosophila.
| Target | Microphthalmia-associated transcription factor (gene MITF; UniProt O75030, human) |
|---|---|
| Clone | AOED-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.8 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human miTF.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microphthalmia-associated transcription factor in the nucleus, cytoplasm and lysosome membrane. Tissue expression noted by UniProt (human): Expressed in melanocytes (at protein level)...
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
