| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32;MITF;BHLHE32; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human MiTF |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MiTF Rabbit Monoclonal Antibody is an antibody targeting MITF. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOED-13; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 75 kDa; calculated MW: 58795 MW.
Boster Bio Anti-MiTF Rabbit Monoclonal Antibody catalog # M00269-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: MITF — Microphthalmia-associated transcription factor
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOED-13; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 75 kDa; Calculated: 58795 MW
Biological background
Protein function (datasheet): Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. .
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines. .
Research relevance and current trends
- Commonly studied in contexts related to 2339,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Immunology,Innate Immunity,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
