| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fzo homolog antibody|Hfzo1 antibody|Hfzo2 antibody|MFN 1 antibody|Mfn1 antibody|MFN1_HUMAN antibody|Mitochondrial transmembrane GTPase Fzo 1 antibody|Mitochondrial transmembrane GTPase FZO 2 antibody|Mitochondrial transmembrane GTPaseFZO1B antibody|Mitofusin 1 antibody|Mitofusin-1 antibody|Mitofusin1 antibody|Putative transmembrane GTPase antibody|Transmembrane GTPase MFN1 antibody |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) is an antibody for MFN1 detection raised in Mouse (Monoclonal, clone Clone: 3H3, Mouse IgG2b), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MFN1 (mitofusin 1); UniProt: Q8IWA4
- Antibody format: Mouse, Monoclonal, clone Clone: 3H3, Mouse IgG2b
- Molecular weight: 84 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) catalog # M02172-1.
Biological background
Biological context: Essential transmembrane GTPase,which mediates mitochondrial fusion.Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology,which is balanced between fusion and fission.MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
Expression and localization notes: cellular localization: Mitochondrion outer membrane ; Multi- pass membrane protein ., tissue context: Ubiquitous.Expressed at slightly higher level in kidney and heart.Isoform 2 may be overexpressed in some tumors,such as lung cancers..
Common research applications
- Western blotting (WB): Compare MFN1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MFN1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify MFN1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80-90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN-2, and to undergo ubiquitination by MARCH5. MFN-1 has two key domains. One is a coiled-coil region that mediates MFN-1: MFN-1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion outer membrane ; Multi- pass membrane protein .
- Tissue details: Ubiquitous.Expressed at slightly higher level in kidney and heart.Isoform 2 may be overexpressed in some tumors,such as lung cancers.
- Research category: Class I,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
