| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kinesin-like protein KIF23;Kinesin-like protein 5;Mitotic kinesin-like protein 1;KIF23;KNSL5, MKLP1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human MKLP1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MKLP1 KIF23 Rabbit Monoclonal Antibody is an antibody targeting KIF23. Common applications include WB, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: EG-11; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 106 kDa; calculated MW: 110059 MW.
Boster Bio Anti-MKLP1 KIF23 Rabbit Monoclonal Antibody catalog # M03483. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: KIF23 — Kinesin-like protein KIF23
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: EG-11; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 106 kDa; Calculated: 110059 MW
Biological background
Protein function (datasheet): Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-ed motor enzyme that moves antiparallel microtubules in vitro. .
Cellular localization (datasheet): Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody. Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.
Tissue details (datasheet): Expressed in the normal brain and also in malignant gliomas (at protein level). .
Research relevance and current trends
- Commonly studied in contexts related to Cardiogenesis,Cardiovascular,Cell Biology,Cell Cycle,Cell Division,Cytoskeleton,Cytoskeleton/ECM,Motor Proteins,Signal Transduction,Spindle.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
