| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kinesin-like protein KIF23;Kinesin-like protein 5;Mitotic kinesin-like protein 1;KIF23;KNSL5, MKLP1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MKLP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EG-11 is a rabbit monoclonal antibody that recognizes kinesin-like protein KIF23 in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 110.1 kDa, and the supplier reports an observed band at about 98 kDa.
KIF23 (MKLP1) is a kinesin-6 motor that, together with the GTPase-activating protein RACGAP1, forms the centralspindlin complex. Centralspindlin bundles microtubules of the central spindle, recruits the Rho activator ECT2, and helps position the contractile ring and form the midbody during cytokinesis. KIF23 is studied in cell division and cancer cell proliferation.
| Target | Kinesin-like protein KIF23 (gene KIF23; UniProt Q02241, human) |
|---|---|
| Clone | EG-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 110.1 kDa |
| Observed MW | 98 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human SiHa cells, human Hacat cells lysate, human U251 cells, rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MKLP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places kinesin-like protein KIF23 in the nucleus, cytoplasm and midbody. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
