| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1; COCA2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MLH1 recombinant protein (Position: S108-C756). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA mismatch repair protein Mlh1 in human samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 84.6 kDa, and the supplier reports an observed band at about 85 kDa.
MLH1 is a core DNA mismatch repair protein. With PMS2 it forms MutLα, which is recruited after MutS complexes find mismatches and helps direct removal of the faulty strand. Loss of MLH1 causes microsatellite instability, and germline mutations are a leading cause of Lynch syndrome, so MLH1 is widely studied in colorectal and endometrial cancer.
| Target | DNA mismatch repair protein Mlh1 (gene MLH1; UniProt P40692, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 84.6 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Daudi cells, human T-47D cells, human MOLT-4 cells, human K562 cells, human U251 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human laryngeal squamous cells carcinoma tissue, human bladder epithelial carcinoma tissue, human colonic adenocarcinoma tissue, human ovarian cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human MLH1 fragment (Ser108–Cys756), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA mismatch repair protein Mlh1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
