| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RecQ-mediated genome instability protein 2; hRMI2; BLM-associated protein of 18 kDa; BLAP18; RMI2; C16orf75 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MLX-interacting protein/MLXIP recombinant protein (Position: H88-P348). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MLX-interacting protein/MLXIP Antibody Picoband® is an antibody for MLXIP detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MLXIP (RecQ mediated genome instability 2); UniProt: Q9HAP2
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 100 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MLX-interacting protein/MLXIP Antibody Picoband® catalog # A08709-2.
Biological background
Biological context: Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. Essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
Expression and localization notes: cellular localization: Nucleus..
Common research applications
- Western blotting (WB): Compare MLXIP levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MLXIP in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify MLXIP-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: This gene encodes a protein that functions as part of a heterodimer to activate transcription. The encoded protein forms a heterodimer with Max-like protein X (MLX) and is involved in the regulation of genes in response to cellular glucose levels.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Research category: Chromatin Binding Proteins,DNA/RNA,DNA/RNA Binding,DNA Damage & Repair,Epigenetics and Nuclear Signaling
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
