| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neutrophil collagenase;3.4.24.34;Matrix metalloproteinase-8;MMP-8;PMNL collagenase;PMNL-CL;MMP8;CLG1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MMP-8, different from the related mouse sequence by eleven amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MMP8 (Neutrophil collagenase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-MMP8 Antibody Picoband® catalog # PB9726. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human MMP-8, different from the related mouse sequence by eleven amino acids.
- Molecular weight context: reported MW: 60 kDa; calculated MW: 53412 MW
- Reactivity: Human
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Neutrophil collagenase; Neutrophil collagenase. MMP8 (Matrix metalloproteinase 8) is a member of the family of matrix metalloproteinases. It is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8 is mapped to 11q21-q22. MMP8 is an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The enzyme exhibits 58% homology to human fibroblast collagenase and has the same domain structure. It consists of a 20-residue signal peptide, and an 80-residue propeptide that is lost on autolytic activation by cleavage of an M-L bond. MMP8 was found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Matrix metalloproteinases (MMPs) have fundamental roles in tumor progression, but most clinical trials with MMP inhibitors have not shown improvements in individuals with cancer. MMP8 has a paradoxical protective role in cancer and provides a genetic model to evaluate the molecular basis of gender differences in cancer susceptibility. Functional note: Can degrade fibrillar type I, II, and III collagens. Reported localization: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules. Expression/tissue context: Neutrophils.
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how MMP8 (Neutrophil collagenase) relates to this theme using model systems and orthogonal readouts.
- Atherosclerosis: Researchers commonly examine how MMP8 (Neutrophil collagenase) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how MMP8 (Neutrophil collagenase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MMP8 (Neutrophil collagenase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of MMP8 (Neutrophil collagenase) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
