| Field | Specification |
|---|---|
| Target | |
| Alternative names | Amine oxidase [flavin-containing] A;1.4.3.4;Monoamine oxidase type A;MAO-A;MAOA; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MAOA, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets amine oxidase (gene MAOA) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 59.7 kDa, and the supplier reports an observed band at about 60 kDa.
Monoamine oxidase A (MAOA) is a flavin-containing enzyme in the outer mitochondrial membrane that deaminates monoamine neurotransmitters, producing hydrogen peroxide. It preferentially breaks down serotonin and norepinephrine and also acts on dopamine, and because it controls neurotransmitter levels it is studied in mood, behavior and the action of MAO inhibitor drugs.
| Target | Amine oxidase (gene MAOA; UniProt P21397, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 59.7 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human RT4 cells, human HepG2 cells, rat liver tissue, rat brain tissue, mouse liver tissue, mouse spinal cord tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse cardiac muscle tissue, rat cardiac muscle tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): U87 cells, U2OS cells.
Immunogen
Synthetic peptide from the C-terminal region of human MAOA. It differs from the mouse sequence at five residues and from the rat sequence at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places amine oxidase in the mitochondrion outer membrane. Tissue expression noted by UniProt (human): Heart, liver, duodenum, blood vessels and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Wei et al. (2026) Systematic toxicology reveals dioctyl terephthalate as a hazardous agent on autism spectrum disorder-like behaviors: Evidence from network toxicology and zebrafish models. Journal of Environmental Sciences. 10.1016/j.jes.2026.03.015
Hang Yang et al. (2025) Aromatherapy With Nardostachys Jatamansi DC. Essential Oil Targeting the MAOA Gene Regulates Cardiac Neural Remodeling and Amino Acid Metabolism to Alleviate Sleep Deprivation-Induced Myocardial Injury. FASEB Journal. 10.1096/fj.202500429R
