| Field | Specification |
|---|---|
| Target | |
| Alternative names | Amine oxidase [flavin-containing] B;1.4.3.4;Monoamine oxidase type B;MAO-B;MAOB; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MAOB, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets amine oxidase (gene MAOB) in human, mouse and rat samples and is validated for immunoprecipitation, IHC and Western blot. The predicted molecular weight is 58.8 kDa, and the supplier reports an observed band at about 59 kDa.
Monoamine oxidase B (MAOB) is a flavin-containing outer mitochondrial membrane enzyme that oxidatively deaminates amines such as phenylethylamine, benzylamine and dopamine, releasing hydrogen peroxide. It converts the neurotoxin MPTP into the toxic metabolite MPP+, and it is studied in dopamine metabolism, astrocyte biology and Parkinson's disease research.
| Target | Amine oxidase (gene MAOB; UniProt P27338, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.8 kDa |
| Observed MW | 59 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
Samples with a confirmed band (WB): human SiHa cells, human HepG2 cells, human HeLa cells, human placenta tissue, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed result (IP): HeLa cells.
Immunogen
Synthetic peptide from the C-terminal region of human MAOB. It differs from the mouse sequence at five residues and from the rat sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places amine oxidase in the mitochondrion outer membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Putnins Edward E. et al. (2021) Monoamine Oxidase-B Inhibitor Reduction in Pro-Inflammatory Cytokines Mediated by Inhibition of cAMP-PKA/EPAC Signaling. Frontiers in Pharmacology. 10.3389/fphar.2021.741460
