| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prolactin regulatory element-binding protein;Mammalian guanine nucleotide exchange factor mSec12;PREB;SEC12; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MOV10 recombinant protein (Position: H167-Q772). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MOV10 Antibody Picoband® is an antibody for MOV10 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MOV10 (Prolactin regulatory element-binding protein); UniProt: Q9HCE1
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 114 kDa, calculated 45468 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MOV10 Antibody Picoband® catalog # A04952.
Biological background
Biological context: Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER. .
Expression and localization notes: cellular localization: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus . Concentrates at endoplasmic reticulum exit sites. ., tissue context: Ubiquitous. ..
Common research applications
- Western blotting (WB): Compare MOV10 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MOV10 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify MOV10-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Putative helicase MOV-10 is an enzyme that in humans is encoded by the MOV10 gene.MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). HumanMOV10may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals. MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection.MOV10 can potently inhibit HIV-1 replication at multiple stages. It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus . Concentrates at endoplasmic reticulum exit sites. .
- Tissue details: Ubiquitous. .
- Research category: Epigenetics and Nuclear Signaling,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
