| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kinesin-like protein KIF20B; Cancer/testis antigen 90; CT90; Kinesin family member 20B; Kinesin-related motor interacting with PIN1; M-phase phosphoprotein 1; MPP1; KIF20B; KRMP1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MPP1/KIF20B recombinant protein (Position: A19-D231). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MPP1/KIF20B Antibody Picoband® is an antibody for KIF20B detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: KIF20B (kinesin family member 20B); UniProt: Q96Q89
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 211 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MPP1/KIF20B Antibody Picoband® catalog # A08340.
Biological background
Biological context: Plus-end-ed motor enzyme that is required for completion of cytokinesis. Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
Expression and localization notes: cellular localization: Nucleus. Nucleolus. Nucleoplasm. Centrosome. Spindle. spindle pole. Midbody. Axon. Growth cone., tissue context: Brain, ovary, kidney and testis. Overexpressed in bladder cancer cells. Expressed in testis. Overexpressed in bladder cancer cells..
Common research applications
- Western blotting (WB): Compare KIF20B levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of KIF20B in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify KIF20B-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Kinesin-like protein KIF20B is a protein that in humans is encoded by the KIF20B gene. The MPP1 gene is previously labeled as KIF20B. Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus. Nucleolus. Nucleoplasm. Centrosome. Spindle. spindle pole. Midbody. Axon. Growth cone.
- Tissue details: Brain, ovary, kidney and testis. Overexpressed in bladder cancer cells. Expressed in testis. Overexpressed in bladder cancer cells.
- Research category: Cell Biology,Cell Cycle,Cell Division,Cytoskeleton,Cytoskeleton/ECM,Motor Proteins,Signal Transduction,Spindle
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
