| Field | Specification |
|---|---|
| Target | |
| Alternative names | MAGUK p55 subfamily member 2;Discs large homolog 2;Protein MPP2;MPP2;DLG2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human MPP2 recombinant protein (Position: R387-Y576). Human MPP2 shares 96.8% amino acid (aa) sequence identity with mouse MPP2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes MAGUK p55 subfamily member 2 (gene MPP2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 64.6 kDa, and the supplier reports an observed band at about 65 kDa.
MPP2 is a MAGUK-family scaffold protein at excitatory synapses. It links synaptic adhesion molecules such as CADM1 to core components of the postsynaptic density and supports synaptic channel function and long-term potentiation in the hippocampus.
| Target | MAGUK p55 subfamily member 2 (gene MPP2; UniProt Q14168, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 64.6 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human MPP2 fragment (Arg387–Tyr576), expressed in E. coli. Sequence identity with the mouse ortholog: 96.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places MAGUK p55 subfamily member 2 in the cytoplasm, membrane, cell projection and postsynaptic density. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
