Anti-MPPB/PMPCB Antibody Picoband®

Overview

Anti-MPPB/PMPCB Antibody Picoband® is an antibody for PMPCB detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: PMPCB (eva-1 homolog A, regulator of programmed cell death); UniProt: O75439
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 43-54 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-MPPB/PMPCB Antibody Picoband® catalog # A11793-1.

Biological background

Biological context: Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.

Expression and localization notes: cellular localization: Endoplasmic reticulum membrane, Single-pass membrane protein, Lysosome membrane., tissue context: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle..

Common research applications

• Western blotting (WB): Compare PMPCB levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of PMPCB in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify PMPCB-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Mitochondrial-processing peptidase subunit beta is an enzyme that in humans is encoded by the PMPCB gene. This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Endoplasmic reticulum membrane, Single-pass membrane protein, Lysosome membrane.
• Tissue details: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.
• Research category: Cell Biology,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,Ubiquitin E3 Enzymes

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.