| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Major histocompatibility complex class I-related gene protein; MHC class I-related gene protein; Class I histocompatibility antigen-like protein; MR1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MR1 recombinant protein (Position: R23-D269). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MR1 Antibody Picoband® is an antibody for MR1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MR1 (major histocompatibility complex, class I-related); UniProt: Q95460
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 40 kDa, calculated 57371 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MR1 Antibody catalog # A00618-1.
Biological background
Biological context: Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent.
Expression and localization notes: cellular localization: Cell membrane. Single-pass membrane protein. Extracellular side. Endoplasmic reticulum., tissue context: Ubiquitous..
Common research applications
- Western blotting (WB): Compare MR1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MR1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify MR1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Major histocompatibility complex class I-related gene protein is a protein that in humans is encoded by the MR1 gene. MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Single-pass membrane protein. Extracellular side. Endoplasmic reticulum.
- Tissue details: Ubiquitous.
- Research category: Calcium Binding Proteins,Calcium Signaling,Cell Biology,Cell Cycle,Cell Differentiation,Cytoskeleton/ECM,Developmental Biology,ECM Proteins,Extracellular Matrix,Organogenesis,Signal Transduction,Signaling Pathway,Skeletal Development,Structures
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
