Anti-MRC1/Cd206 Rabbit Monoclonal Antibody

SKU:BHA21007945
Research Validated
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-MRC1 antibody (Rabbit monoclonal, clone AOOF-13, Rabbit IgG). Validated for Western blot and immunofluorescence. Reactivity: Human. Recognizes macrophage mannose receptor 1. Observed band in Western blot: about 166 kDa.
Target MRC1
Clone number AOOF-13
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, ICC, IF
Options selector
Catalog no. Size Conjugation
M02285 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB,ICC,IF
      Application details: WB 1:500-2000
      ICC/IF 1:50-200
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MRC1
Alternative names Macrophage mannose receptor 1;MMR;C-type lectin domain family 13 member D;C-type lectin domain family 13 member D-like;Human mannose receptor;hMR;Macrophage mannose receptor 1-like protein 1;CD206;MRC1;CLEC13D, CLEC13DL, MRC1L1;
Gene ID 4360
UniProt # P22897
Host Rabbit
Clone AOOF-13
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunofluorescence
Immunogen A synthesized peptide derived from human MRC1
Molecular weight 166.0 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Endosome membrane ; Single-pass type I membrane protein . Cell membrane ; Single-pass type I membrane protein .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M02285
Main SKU BHA21007945
Rabbit Monoclonal

Product Overview

Clone AOOF-13 is a rabbit monoclonal antibody that recognizes macrophage mannose receptor 1 (gene MRC1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 166.0 kDa, and the supplier reports an observed band at about 166 kDa.

The mannose receptor (MRC1, CD206) is a C-type lectin receptor on macrophages and dendritic cells. It binds and internalizes glycoproteins and microbes carrying mannose and related sugars, and CD206 is widely used as a marker of alternatively activated (M2-like) macrophages.

Target Macrophage mannose receptor 1 (gene MRC1; UniProt P22897, human)
Clone AOOF-13
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 166.0 kDa
Observed MW 166 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200

Immunogen

Synthetic peptide based on the sequence of human MRC1.

Reactivity Notes

The supplier lists reactivity with human. UniProt places macrophage mannose receptor 1 in the endosome membrane and cell membrane.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 166.0 kDa; observed band at about 166 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human MRC1.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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