| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Multidrug resistance-associated protein 1;ATP-binding cassette sub-family C member 1;Leukotriene C (4) transporter;LTC4 transporter;ABCC1;MRP, MRP1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MRP1/ABCC1 recombinant protein (Position: S239-K357). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MRP1/ABCC1 Antibody Picoband® is an antibody for ABCC1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ABCC1 (Multidrug resistance-associated protein 1); UniProt: P33527
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 220 kDa, calculated 171591 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MRP1/ABCC1 Antibody Picoband® catalog # A00872-1.
Biological background
Biological context: Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o- glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. .
Expression and localization notes: cellular localization: Cell membrane ; Multi-pass membrane protein ., tissue context: Lung, testis and peripheral blood mononuclear cells..
Common research applications
- Western blotting (WB): Compare ABCC1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ABCC1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ABCC1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Multidrug resistance-associated protein 1 (MRP1) is a protein that in humans is encoded by the ABCC1 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane ; Multi-pass membrane protein .
- Tissue details: Lung, testis and peripheral blood mononuclear cells.
- Research category: ATPases,Cancer,Channels,Drug Metabolism,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Plasma Membrane,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
