| Field | Specification |
|---|---|
| Target | |
| Alternative names | Multidrug resistance-associated protein 4;ATP-binding cassette sub-family C member 4;MRP/cMOAT-related ABC transporter;Multi-specific organic anion transporter B;MOAT-B;ABCC4;MRP4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MRP4 recombinant protein (Position: M1-P370). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ATP-binding cassette sub-family C member 4 (gene ABCC4) in rat, mouse and human samples and is validated for IHC and Western blot. The predicted molecular weight is 149.5 kDa, and the supplier reports an observed band at about 47 kDa.
MRP4 (ABCC4) is an ATP-dependent efflux pump that exports signaling molecules such as cAMP, cGMP and prostaglandins as well as bile acids, urate and many drugs. It is expressed in kidney, platelets and other tissues and is studied in cell signaling and drug disposition.
| Target | ATP-binding cassette sub-family C member 4 (gene ABCC4; UniProt O15439, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 149.5 kDa |
| Observed MW | 47 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue, human intestinal cancer tissue, mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human MRP4 fragment (Met1–Pro370), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places ATP-binding cassette sub-family C member 4 in the basolateral cell membrane and apical cell membrane. Tissue expression noted by UniProt (human): Widely expressed, with particularly high levels in prostate, but is barely detectable in liver. sinusoidal membrane of hepatocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Songsong Wang et al. (2021) The Herbal Constituents in An-Gong-Niu-Huang Wan (AGNH) Protect against Cinnabar- and Realgar-Induced Hepatorenal Toxicity and Accumulations of Mercury and Arsenic in Mice. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/5566078
Chen Lin et al. (2018) Influence of Orally Administered Borneol on the Expression of Hepatic Transporters in Rats. European Journal of Drug Metabolism and Pharmacokinetics. 10.1007/s13318-018-0499-1
