| Field | Specification |
|---|---|
| Alternative Names | HNPCC, HNPCC1 |
| Clonality | |
| Concentration | |
| Formulation | |
| Immunogen | Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3). |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Storage |
Product Overview
Research Use OnlyMSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma.
Validated Applications
| Western Blot (WB) | See datasheet for recommended dilution |
|---|---|
| Immunohistochemistry (IHC) | See datasheet for recommended dilution |
| ELISA (ELISA) | See datasheet for recommended dilution |
Key Specifications
| Clonality | Monoclonal |
|---|---|
| Species Reactivity | Human |
| Isotype | IgG1 |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Catalog No. | Y050845 |
| Alternative Names | HNPCC, HNPCC1 |
Immunogen / Antigen
Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
Reactivity Notes
This antibody has been validated for reactivity against Human targets. Cross-reactivity with other species may exist but has not been formally established.
Storage & Handling
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunohistochemistry, ELISA. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
Recommended dilutions vary by application and sample type. Start with the manufacturer's suggested range (typically 1:500–1:2000 for WB; 1:50–1:200 for IHC/IF) and optimize for your specific experimental conditions.
This antibody has been confirmed reactive against: Human. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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