| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA mismatch repair protein Msh6 ;hMSH6 ;G/T mismatch-binding protein ;GTBP ;GTMBP ;MutS protein homolog 6 ;MutS-alpha 160 kDa subunit;p160 ;MSH6 ;GTBP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MSH6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEB-13 is a rabbit monoclonal antibody that recognizes DNA mismatch repair protein Msh6 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 152.8 kDa, and the supplier reports an observed band at about 160 kDa.
MSH6 is a DNA mismatch repair protein that pairs with MSH2 to form MutSα, the main sensor of single-base mismatches and small insertion-deletion loops after DNA replication. Loss of MSH6 impairs mismatch repair, and germline mutations are one cause of Lynch syndrome.
| Target | DNA mismatch repair protein Msh6 (gene MSH6; UniProt P52701, human) |
|---|---|
| Clone | AEB-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 152.8 kDa |
| Observed MW | 160 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human K562 cells, human HeLa cells, rat brain tissue, rat RH35 cells, mouse brain tissue, mouse NIH/3T3 cells, human A549- WT cells, human A549-MSH6 KO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human esophageal cancer tissue, human ovarian cancer tissu, human tonsil tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human MSH6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA mismatch repair protein Msh6 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
