| Field | Specification |
|---|---|
| Target | |
| Alternative names | Paired amphipathic helix protein Sin3a; Histone deacetylase complex subunit Sin3a; Transcriptional corepressor Sin3a; SIN3A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human mSin3A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACEE-19 is a rabbit monoclonal antibody that recognizes paired amphipathic helix protein Sin3a in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 145.2 kDa, and the supplier reports an observed band at about 150 kDa.
SIN3A is a large scaffold protein at the core of the SIN3–HDAC corepressor complex, which also contains HDAC1 and HDAC2. Recruited by repressors such as MAD/MXI1–MAX and REST, it silences target genes through histone deacetylation, and it takes part in MYC antagonism, circadian gene repression and neural development.
| Target | Paired amphipathic helix protein Sin3a (gene SIN3A; UniProt Q96ST3, human) |
|---|---|
| Clone | ACEE-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 145.2 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells, RAW 2647 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human mSin3A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places paired amphipathic helix protein Sin3a in the nucleus. Tissue expression noted by UniProt (human): Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jie Ren et al. (2019) miR‑210‑3p regulates the proliferation and apoptosis of non‑small cell lung cancer cells by targeting SIN3A. Experimental and Therapeutic Medicine. 10.3892/etm.2019.7867
