Anti-mSin3A Rabbit Monoclonal Antibody

Overview

This product is an anti-SIN3A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ACEE-19; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-mSin3A Rabbit Monoclonal Antibody catalog # M01203. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: SIN3A (CD9 antigen).
• Antibody format: Monoclonal; clone ACEE-19; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

SIN3A (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. . Reported cellular localization context: Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Tissue expression notes (as provided): Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells. .

Research relevance and current trends

• Research context keywords from the source record include: 2339,Co-Factors,Cofactors, Vitamins/Minerals,Epigenetics and Nuclear Signaling,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Transcription.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate SIN3A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SIN3A expression by Western blot in cell or tissue lysates, Detect SIN3A in FFPE tissue sections by immunohistochemistry, Localize SIN3A by immunofluorescence/immunocytochemistry in cultured cells, Quantify SIN3A-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 17 kDa; calculated MW: 25416 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 17 kDa
• Cellular localization (provided): Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein .
• Tissue details (provided): Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.