| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADH-ubiquinone oxidoreductase chain 4; NADH dehydrogenase subunit 4; MT-ND4; MTND4; NADH4; ND4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NADH-ubiquinone oxidoreductase chain 4 (gene MT-ND4) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 51.6 kDa, and the supplier reports an observed band at about 35–40 kDa.
MT-ND4 is a mitochondrial DNA-encoded core subunit of the membrane arm of complex I (NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain. It contributes to proton pumping and complex I activity, and the m.11778G>A mutation in MT-ND4 is the most common cause of Leber hereditary optic neuropathy.
| Target | NADH-ubiquinone oxidoreductase chain 4 (gene MT-ND4; UniProt P03905, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 51.6 kDa |
| Observed MW | 35–40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, human Caco-2 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human MT-ND4 fragment (Met10–Ser459), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NADH-ubiquinone oxidoreductase chain 4 in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
