| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Rho-related GTP-binding protein Rho6;Rho family GTPase 1;Rnd1;RND1;RHO6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MT2-MMP/MMP15 recombinant protein (Position: Y132-A202). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MT2-MMP/MMP15 Antibody Picoband® is an antibody for MMP15 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MMP15 (Rho-related GTP-binding protein Rho6); UniProt: P51511
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 74 kDa, calculated 26056 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MT2-MMP/MMP15 Antibody Picoband® catalog # A06396-1.
Biological background
Biological context: Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. .
Expression and localization notes: cellular localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton ., tissue context: Mostly expressed in brain and liver..
Common research applications
- Western blotting (WB): Compare MMP15 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MMP15 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify MMP15-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Matrix metalloproteinase 15 also known as MMP15 is an enzyme that in humans is encoded by the MMP15 gene. This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein may play a role in cancer progression.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton .
- Tissue details: Mostly expressed in brain and liver.
- Research category: Actin Assembly,Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,G Protein Signaling,Microfilaments,Ras Family,Signal Transduction,Signaling Pathway,Small G Proteins
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
