| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human mTOR/MTOR recombinant protein (Position: N2093-N2537). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets serine/threonine-protein kinase mTOR in human and monkey samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 288.9 kDa, and the supplier reports an observed band at about 289 kDa.
mTOR is a serine/threonine kinase that forms two complexes, mTORC1 and mTORC2, and serves as a central integrator of nutrient, growth factor and energy signals. mTORC1 drives protein, lipid and nucleotide synthesis through targets such as S6K and 4E-BP1 and suppresses autophagy. mTOR signaling is studied across cell growth, metabolism, aging, immunity and cancer.
| Target | Serine/threonine-protein kinase mTOR (gene MTOR; UniProt P42345, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 288.9 kDa |
| Observed MW | 289 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HEK293 cells, human HeLa cells, monkey COS-7 cells, human A431 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human mTOR/MTOR fragment (Asn2093–Asn2537), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and monkey. UniProt places serine/threonine-protein kinase mTOR in the lysosome membrane, endoplasmic reticulum membrane and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Expressed in numerous tissues, with highest levels in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lu Kong et al. (2021) Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells. Environmental Pollution. 10.1016/j.envpol.2021.116556
Xinying Zhang et al. (2021) Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway. International Journal of Molecular Medicine. 10.3892/ijmm.2021.4860
Peng Wang et al. (2019) ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro. Toxicology and Applied Pharmacology. 10.1016/j.taap.2019.114647
Juan Bai et al. (2026) Momordicine I Mediates Lipid-Lowering Effects of Momordica Charantia L. Triterpenoid Saponins via TFEB-Driven Lysosomal Biogenesis. Journal of Food Science. 10.1111/1750-3841.70923
Liangui Nie et al. (2021) Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway. Molecular Medicine Reports. 10.3892/mmr.2021.11938
Xiao‑Xiang Zhai et al. (2017) Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts. Molecular Medicine Reports. 10.3892/mmr.2017.6437
