| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor A, mitochondrial;mtTFA;Mitochondrial transcription factor 1;MtTF1;Transcription factor 6;TCF-6;Transcription factor 6-like 2;TFAM;TCF6, TCF6L2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human mtTFA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets transcription factor A, mitochondrial (gene TFAM) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 29.1 kDa, and the supplier reports an observed band at about 24 kDa.
Mitochondrial transcription factor A (TFAM) is a DNA-binding protein that packages mitochondrial DNA into nucleoids. It also recruits the mitochondrial RNA polymerase POLRMT to promoters, together with TFB2M, so it controls both mitochondrial transcription and mtDNA copy number and is widely used as a marker of mitochondrial biogenesis.
| Target | Transcription factor A, mitochondrial (gene TFAM; UniProt Q00059, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 29.1 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human 293T cells, human K562 cells, human Caco-2 cells, huamn Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human intestinal diffuse large B-cells lymphoma tissue, human ovarian serous adenocarcinoma tissue, human tonsil tissue, human liver cancer tissue, human lung adenocarcinoma tissue, human colorectal adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Synthetic peptide from the C-terminal region of human mtTFA.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcription factor A, mitochondrial in the mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fan Wenlu et al. (2021) FARS2 deficiency in Drosophila reveals the developmental delay and seizure manifested by aberrant mitochondrial tRNA metabolism. Nucleic Acids Research. 10.1093/nar/gkab1187
