| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transcription factor A, mitochondrial; mtTFA; Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2; TFAM; TCF6, TCF6L2 |
| Cellular Localization | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA, different from the related mouse and rat sequences by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-mtTFA TFAM Antibody Picoband® (monoclonal, 4D9) is an antibody for TFAM detection raised in Mouse (Monoclonal, clone Clone: 4D9, Mouse IgG2b), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: TFAM (transcription factor A, mitochondrial); UniProt: Q00059
- Antibody format: Mouse, Monoclonal, clone Clone: 4D9, Mouse IgG2b
- Molecular weight: 40 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-mtTFA TFAM Antibody Picoband® (monoclonal, 4D9) catalog # M01119-1.
Biological background
Biological context: Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.
Expression and localization notes: cellular localization: Mitochondrion; mitochondrion nucleoid, tissue context: Widely expressed at low level. Expressed in heart,brain,skeletal muscle,placenta,pancreas,spleen,prostate,mall intestine,colon,peripheral blood,leukocytes,bone marrow and fetal liver.Highly expressed in testes..
Common research applications
- Western blotting (WB): Compare TFAM levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of TFAM in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify TFAM-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion; mitochondrion nucleoid
- Tissue details: Widely expressed at low level. Expressed in heart,brain,skeletal muscle,placenta,pancreas,spleen,prostate,mall intestine,colon,peripheral blood,leukocytes,bone marrow and fetal liver.Highly expressed in testes.
- Research category: G Protein Signaling,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
