| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-2; MUC-2; Intestinal mucin-2; MUC2; SMUC |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MUC2, which shares 86.1% amino acid (aa) sequence identity with both mouse and rat MUC2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mucin-2 (gene MUC2) in human, mouse and rat samples and is validated for ICC/IF and IHC. The predicted molecular weight is 540.3 kDa.
Mucin-2 (MUC2) is the main gel-forming mucin secreted by intestinal goblet cells. Its large polymeric networks build the colonic mucus layer that separates bacteria from the epithelium while allowing nutrient uptake, and it has also been shown to bind copper. MUC2 is a standard goblet cell marker in studies of gut barrier function, colitis and the microbiome.
| Target | Mucin-2 (gene MUC2; UniProt Q02817, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 540.3 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human, Mouse) |
Samples with confirmed staining (IHC): human colon cancer tissue, mouse colon tissue, rat colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human ileum tissue, human colon organoid tissue, mouse ileum tissue, mouse ileum organoid tissue, human intestine cancer tissue, mouse intestine tissue.
Immunogen
Synthetic peptide from the N-terminal region of human MUC2. Sequence identity with the mouse and rat orthologs: 86.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes mucin-2 as a secreted protein. Tissue expression noted by UniProt (human): Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan Chen et al. (2025) Defining enteric bacterial pathogenesis using organoids: Citrobacter rodentium uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus. Gut Microbes. 10.1080/19490976.2025.2494717
Caiyun Wang et al. (2021) Maternal exposure to sodium ρ-perfluorous nonenoxybenzene sulfonate during pregnancy and lactation disrupts intestinal barrier and may cause obstacles to the nutrient transport and metabolism in F0 and F1 generations of mice. Science of the Total Environment. 10.1016/j.scitotenv.2021.148775
Meng-Meng Niu et al. (2026) Bifidobacterium bifidum Alleviate Intestinal Barrier Damage by Regulating the Intestinal Flora and Colonic Mucin O-Glycan Structural Patterns. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.5c12182
Luo Pan et al. (2022) Central administration of human opiorphin alleviates dextran sodium sulfate-induced colitis in mice through activation of the endogenous opioid system. Frontiers in Pharmacology. 10.3389/fphar.2022.904926
Zohreh Sharafian et al. (2025) Crosstalk with infant-derived Th17 cells, as well as exposure to IL-22 promotes maturation of intestinal epithelial cells in an enteroid model. Frontiers in Immunology. 10.3389/fimmu.2025.1582688
Zihong Pan et al. (2019) Subchronic exposure of environmentally relevant concentrations of F-53B in mice resulted in gut barrier dysfunction and colonic inflammation in a sex-independent manner. Environmental Pollution. 10.1016/j.envpol.2019.07.021
Jan Homolak et al. (2023) Altered Secretion, Constitution, and Functional Properties of the Gastrointestinal Mucus in a Rat Model of Sporadic Alzheimer’s Disease. ACS Chemical Neuroscience. 10.1021/acschemneuro.3c00223
Sadia Kanwal et al. (2018) A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model. Nutrients. 10.3390/nu10081003
Xiaozhu Tian et al. (2020) Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake. Journal of Functional Foods. 10.1016/j.jff.2020.104045
Xinxiu Ren et al. (2018) Polysaccharide extracted from Enteromorpha ameliorates Cisplastin-induced small intestine injury in mice. Journal of Functional Foods. 10.1016/j.jff.2018.08.023
