| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein scribble homolog; Scribble; hScrib; Protein LAP4; SCRIB; CRIB1; KIAA0147; LAP4; SCRB1; VARTUL |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MUC4 recombinant protein (Position: Q1250-D1444). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MUC4 Antibody Picoband® is an antibody for MUC4 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MUC4 (scribbled planar cell polarity protein); UniProt: Q99102
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 170 kDa, calculated 50514 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-MUC4 Antibody Picoband® catalog # A01652-2.
Biological background
Biological context: Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
Expression and localization notes: cellular localization: Cell membrane., tissue context: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level)..
Common research applications
- Western blotting (WB): Compare MUC4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MUC4 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify MUC4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Mucin 4, also known as MUC4, is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, Mucin 4 is a high-molecular weight glycoprotein. It is mapped to 3q29. In normal functioning, Mucin 4 is known to play anti-adhesive roles in the body, such as in lubricating the reproductive lining. It is found in the respiratory tract, particularly in the trachea and lung and the digestive tract, in the esophagus and colon, as well as in the visual and auditory systems. Mucin 4 serves to protect and lubricate the epithelium, which facilitates transport and traps foreign particles. It is found to be overexpressed during the luteal phase of menstruation. Mucin 4 may also have a role in fetal morphogenic development.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane.
- Tissue details: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
- Research category: Microbiology
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
