| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-5B; MUC-5B; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B, tracheobronchial; Sublingual gland mucin; MUC5B; MUC5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MUC5B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFCO-13 is a rabbit monoclonal antibody that recognizes mucin-5B (gene MUC5B) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 596.3 kDa, and the supplier reports an observed band at about 596 kDa.
Mucin-5B (MUC5B) is a gel-forming mucin secreted in the airways, salivary glands and cervix. It contributes to the viscoelastic properties of saliva and cervical mucus and is essential for mucociliary clearance in the lungs. A common promoter variant is a well-known risk factor for idiopathic pulmonary fibrosis.
| Target | Mucin-5B (gene MUC5B; UniProt Q9HC84, human) |
|---|---|
| Clone | AFCO-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 596.3 kDa |
| Observed MW | 596 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MUC5B.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes mucin-5B as a secreted protein. Tissue expression noted by UniProt (human): Expressed on surface airway epithelia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
