| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Mucin from human ovarian cyst fluid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) is an antibody targeting MUC5B. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: 45M1; isotype: Mouse IgG1; reactivity: Human,Rabbit,Rat; observed MW: 200 kDa; calculated MW: 585570 MW.
Boster Bio Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) catalog # MA1061. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rabbit, Rat.
Key elements and design rationale
- Target: MUC5B — Mucin-5AC
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: 45M1; Isotype: Mouse IgG1
- Species reactivity: Human,Rabbit,Rat
- Molecular weight guidance: Observed: 200 kDa; Calculated: 585570 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
Scientific background (datasheet): Mucin 5AC (Muc5AC) is a protein that in humans is encoded by the MUC5AC gene. Using real-time RT-PCR, Moehle et al. (2006) found that MUC5AC was highly expressed in adult trachea and stomach. Expression was lower in adult lung and weak in fetal lung, and no expression was detected in other tissues. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease. Moreover, Muc5ac is identified as a and critical mediator of resistance during intestinal nematode infection.
Cellular localization (datasheet): Secreted .
Tissue details (datasheet): Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Sequence similarities (datasheet): Contains 1 CTCK (C-terminal cystine knot-like) domain.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Tumor Antigens,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
