| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myeloid differentiation primary response protein MyD88; Myd88; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MYD88 recombinant protein (Position: R62-P296). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes myeloid differentiation primary response protein MyD88 in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 33.2 kDa, and the supplier reports an observed band at about 37 kDa.
MyD88 is the adaptor that links Toll-like receptors and IL-1 family receptors to downstream kinases in innate immunity. Through IRAK1, IRAK2 and TRAF6 it drives NF-κB activation and the release of inflammatory cytokines, so it is a common readout in studies of infection, sterile inflammation and TLR-targeted drugs.
| Target | Myeloid differentiation primary response protein MyD88 (gene MYD88; UniProt Q99836, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 33.2 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human K562 cells, human A549 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human MYD88 fragment (Arg62–Pro296), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places myeloid differentiation primary response protein MyD88 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huan Yang et al. (2014) Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via Lipopolysaccharide Stimulation and Is Overexpressed in Patients with Lymph Node Metastasis. PLoS One. 10.1371/journal.pone.0109980
Long Yue et al. (2016) Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2016.05.002
Wang Lu et al. (2015) High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy. Diabetology & Metabolic Syndrome. 10.1186/s13098-015-0086-4
Xiaohui Jin et al. (2020) Porcine parvovirus nonstructural protein NS1 activates NF-κB and it involves TLR2 signaling pathway. Journal of Veterinary Science. 10.4142/jvs.2020.21.e50
