| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Peptidyl-prolyl cis-trans isomerase B;PPIase B;5.2.1.8;CYP-S1;Cyclophilin B;Rotamase B;S-cyclophilin;SCYLP;PPIB;CYPB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Myogenin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MYOG antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFFH-13; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, Flow (as provided in the source record). Boster Bio Anti-Myogenin Monoclonal Antibody catalog # M03238-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: MYOG (Peptidyl-prolyl cis-trans isomerase B).
- Antibody format: Monoclonal; clone AFFH-13; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MYOG (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Reported cellular localization context: Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue expression notes (as provided): Expressed in brain in the thalamus, in fetal and adult lung and in placenta. .
Research relevance and current trends
- Research context keywords from the source record include: Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Mesenchymal Stem Cells,Stem Cells,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate MYOG antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MYOG expression by Western blot in cell or tissue lysates, Detect MYOG in FFPE tissue sections by immunohistochemistry, Quantify MYOG-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 71 kDa; calculated MW: 23743 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 71 kDa
- Cellular localization (provided): Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Tissue details (provided): Expressed in brain in the thalamus, in fetal and adult lung and in placenta. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
