| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4; MYOG; MYF4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Myogenin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFFH-13 is a rabbit monoclonal antibody that recognizes myogenin in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 25.0 kDa, and the supplier reports an observed band at about 36 kDa.
Myogenin (MYOG) is a MyoD-family bHLH transcription factor that drives the terminal differentiation of myoblasts into myotubes. It is essential for embryonic skeletal muscle formation and is a standard marker of differentiating muscle cells and of rhabdomyosarcoma in research.
| Target | Myogenin (gene MYOG; UniProt P15173, human) |
|---|---|
| Clone | AFFH-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 25.0 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:40 |
Samples with a confirmed band (WB): C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human myogenin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places myogenin in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gloria Buffi et al. (2025) Myotube-specific vulnerability to clozapine. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2025.118661
Lan Ma et al. (2023) Fluoride regulates the differentiation and atrophy through FGF21/ERK signaling pathway in C2C12 cells. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2023.114626
Zhang Rong-Ping et al. (2013) Silencing Pax3 by shRNA inhibits the proliferation and differentiation of duck (Anas platyrhynchos) myoblasts. Molecular and Cellular Biochemistry. 10.1007/s11010-013-1859-4
