| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform ;MLC-2 ;MLC-2v;Cardiac myosin light chain 2 ;Myosin light chain 2, slow skeletal/ventricular muscle isoform ;MLC-2s/v ;Ventricular myosin light chain 2 ;MYL2 ;MLC2 ; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Myosin Light Chain 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FHO-13 is a rabbit monoclonal antibody that recognizes myosin regulatory light chain 2, ventricular/cardiac muscle isoform (gene MYL2) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 18.8 kDa, and the supplier reports an observed band at about 18 kDa.
MLC-2v (MYL2) is the regulatory light chain of cardiac ventricular myosin. Its phosphorylation increases contractile force and calcium sensitivity, and it supports myofibril assembly during heart development. MYL2 is a standard ventricular cardiomyocyte marker, and its mutations are linked to hypertrophic cardiomyopathy.
| Target | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (gene MYL2; UniProt P10916, human) |
|---|---|
| Clone | FHO-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 18.8 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with confirmed staining (IHC): human heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human myosin Light Chain 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places myosin regulatory light chain 2, ventricular/cardiac muscle isoform in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in type I muscle fibers. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
