| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NADH-ubiquinone oxidoreductase chain 2; NADH dehydrogenase subunit 2; Mtnd2; mt-Nd2; Nd2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of rat NADH2/Mtnd2, which shares 80% amino acid (aa) sequence identity with mouse NADH2/Mtnd2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NADH2/Mtnd2 Antibody Picoband® is an antibody for Mtnd2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Mtnd2 (NADH dehydrogenase 2, mitochondrial); UniProt: P11662
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 39 kDa, calculated 14377 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-NADH2/Mtnd2 Antibody Picoband® catalog # A32839-1.
Biological background
Biological context: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Expression and localization notes: cellular localization: Mitochondrion inner membrane. Multi-pass membrane protein., tissue context: Ubiquitously expressed..
Common research applications
- Western blotting (WB): Compare Mtnd2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Mtnd2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Mtnd2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Mitochondrially encoded NADH dehydrogenase 2 is protein that in humans is encoded by the mitochondrial gene MT-ND2 gene. The ND2 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of MT-ND2 are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion inner membrane. Multi-pass membrane protein.
- Tissue details: Ubiquitously expressed.
- Research category: Chemokines,Immunology,Innate Immunity
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
