| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase TBK1;2.7.11.1;NF-kappa-B-activating kinase;T2K;TANK-binding kinase 1;TBK1;NAK; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NAK/TBK1 (N-term) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AHE-20 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase TBK1 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 84 kDa.
TBK1 is a serine/threonine kinase that drives type I interferon responses to viral and bacterial infection. Recruited by adaptors such as STING, MAVS and TRIF, it phosphorylates IRF3 and IRF7 so they can enter the nucleus and induce interferon genes, and it also regulates selective autophagy; TBK1 variants are linked to ALS and frontotemporal dementia.
| Target | Serine/threonine-protein kinase TBK1 (gene TBK1; UniProt Q9UHD2, human) |
|---|---|
| Clone | AHE-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.6 kDa |
| Observed MW | 84 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human MCF-7 cells, human K562 cells, rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat liver tissue, rat stomach tissue, human lung tissue, human colon cancer tissue, mouse skin tissue, human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human NAK/TBK1 (N-term).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase TBK1 in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous with higher expression in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tang Ming et al. (2024) The P286R mutation of DNA polymerase ε activates cancer-cell-intrinsic immunity and suppresses endometrial tumorigenesis via the cGAS-STING pathway. Cell Death & Disease. 10.1038/s41419-023-06418-3
Hao Jinfang et al. (2024) TLR4 signalling: the key to controlling EV71 replication and inflammatory response. Frontiers in Cellular and Infection Microbiology. 10.3389/fcimb.2024.1393680
Haomou Pu et al. (2026) Delphinidin, a dietary anthocyanidin, modulates PD-L1 and STING/TBK1 signaling and reprograms the immune microenvironment in triple-negative breast cancer. Journal of Functional Foods. 10.1016/j.jff.2026.107268
