| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleosome assembly protein 1-like 1; Histone H2A-H2B chaperone NAP1L1; NAP-1-related protein; hNRP; NAP1L1; NRP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NAP1L1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23N17 is a rabbit monoclonal antibody that recognizes nucleosome assembly protein 1-like 1 (gene NAP1L1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 45.4 kDa; the supplier reports observed bands at 52, 60 kDa.
NAP1L1 (nucleosome assembly protein 1-like 1) is a histone chaperone that shuttles H2A-H2B dimers into the nucleus and helps assemble and disassemble nucleosomes. Through this activity it influences transcription, chromatin remodeling and DNA repair, and it is studied in chromatin biology and neural development.
| Target | Nucleosome assembly protein 1-like 1 (gene NAP1L1; UniProt P55209, human) |
|---|---|
| Clone | 23N17 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.4 kDa |
| Observed MW | 52, 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NAP1L1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nucleosome assembly protein 1-like 1 in the nucleus, chromosome, melanosome and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
