| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Keratin, type II cytoskeletal 2 epidermal;Cytokeratin-2e;CK-2e;Epithelial keratin-2e;Keratin-2 epidermis;Keratin-2e;K2e;Type-II keratin Kb2;KRT2;KRT2A, KRT2E; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human NAPSIN A May be involved in processing of pneumocyte surfactant precursors. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-NAPSA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFBB-14; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-Napsin A NAPSA Monoclonal Antibody catalog # M05685-2. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: NAPSA (Keratin, type II cytoskeletal 2 epidermal).
- Antibody format: Monoclonal; clone AFBB-14; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
NAPSA (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization. . Reported cellular localization context: Cytoplasm, cytoskeleton. Tissue expression notes (as provided): Expressed in the upper spinous and granular suprabasal layers of normal adult epidermal tissues from most body sites including thigh, breast nipple, foot sole, penile shaft and axilla. Not present in foreskin, squamous metaplasias and carcinomas. Expression in hypertrophic and keloid scars begins in the deepest suprabasal layer. Weakly expressed in normal gingiva and tongue, however expression is induced in benign keratoses of lingual mucosa and in mild-to-moderate oral dysplasia with orthokeratinization. .
Research relevance and current trends
- Research context keywords from the source record include: Cell Biology,Tags & Cell Markers,Proteolysis/Ubiquitin,Cell Type Markers,Tumor Associated,Proteolytic Enzymes.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate NAPSA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NAPSA expression by Western blot in cell or tissue lysates, Detect NAPSA in FFPE tissue sections by immunohistochemistry, Enrich NAPSA by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 160 kDa; calculated MW: 65433 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 160 kDa
- Cellular localization (provided): Cytoplasm, cytoskeleton.
- Tissue details (provided): Expressed in the upper spinous and granular suprabasal layers of normal adult epidermal tissues from most body sites including thigh, breast nipple, foot sole, penile shaft and axilla. Not present in foreskin, squamous metaplasias and carcinomas. Expression in hypertrophic and keloid scars begins in the deepest suprabasal layer. Weakly expressed in normal gingiva and tongue, however expression is induced in benign keratoses of lingual mucosa and in mild-to-moderate oral dysplasia with orthokeratinization. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
