| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Arylamine N-acetyltransferase 1;2.3.1.5;Arylamide acetylase 1;Monomorphic arylamine N-acetyltransferase;MNAT;N-acetyltransferase type 1;NAT-1;NAT1;AAC1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NAT1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of NAT1 (Arylamine N-acetyltransferase 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-NAT1 Antibody Picoband® catalog # A01386-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human NAT1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
- Molecular weight context: reported MW: 34 kDa; calculated MW: 33899 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Arylamine N-acetyltransferase 1; Arylamine N-acetyltransferase 1. N-acetyltransferase 1 (arylamine N-acetyltransferase) is a protein that in humans is encoded by the NAT1 gene. This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. Functional note: Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. Reported localization: Cytoplasm.
Research relevance and current trends
- Amino Acid Metabolism: Researchers commonly examine how NAT1 (Arylamine N-acetyltransferase 1) relates to this theme using model systems and orthogonal readouts.
- Amino Acids: Researchers commonly examine how NAT1 (Arylamine N-acetyltransferase 1) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how NAT1 (Arylamine N-acetyltransferase 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative NAT1 (Arylamine N-acetyltransferase 1) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
