| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calcium-binding and coiled-coil domain-containing protein 2; Antigen nuclear dot 52 kDa protein; Nuclear domain 10 protein NDP52; Nuclear domain 10 protein 52; Nuclear dot protein 52; CALCOCO2; NDP52 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NDP52/CALCOCO2 recombinant protein (Position: M1-L446). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 9E2F2 is a mouse monoclonal antibody that recognizes calcium-binding and coiled-coil domain-containing protein 2 (gene CALCOCO2) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 52.3 kDa, and the supplier reports an observed band at about 52 kDa.
CALCOCO2, better known as NDP52, is a selective autophagy receptor. It recognizes ubiquitin- and galectin-8-coated bacteria that enter the cytosol, such as Salmonella, and links them to LC3 on autophagosome membranes for degradation (xenophagy). NDP52 also contributes to mitophagy, and it is studied in innate immunity and autophagy.
| Target | Calcium-binding and coiled-coil domain-containing protein 2 (gene CALCOCO2; UniProt Q13137, human) |
|---|---|
| Clone | 9E2F2 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.3 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human placenta tissue, human HeLa cells, human Jurkat cells, human A549 cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human NDP52/CALCOCO2 fragment (Met1–Leu446), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places calcium-binding and coiled-coil domain-containing protein 2 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
