| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nebulin;NEB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Chicken breast nebulin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Nebulin Antibody (Monoclonal, NB2) is an antibody targeting NEB. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: NB2; isotype: Mouse IgG1; reactivity: Chicken; observed MW: 773 kDa; calculated MW: 772914 MW.
Boster Bio Anti-Nebulin Antibody (Monoclonal, NB2) catalog # MA1069. Tested in WB applications. This antibody reacts with Chicken.
Key elements and design rationale
- Target: NEB — Nebulin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: NB2; Isotype: Mouse IgG1
- Species reactivity: Chicken
- Molecular weight guidance: Observed: 773 kDa; Calculated: 772914 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
Scientific background (datasheet): Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.
Cellular localization (datasheet): Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Tissue details (datasheet): Muscle specific. Located in the thin filament of striated muscle.
Sequence similarities (datasheet): Contains 178 nebulin repeats.
Research relevance and current trends
- Commonly studied in contexts related to Actin Binding Proteins,Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
