| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;Nefh;Nfh; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NEFH Antibody (Monoclonal, N52) is an antibody targeting NEFH. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: N52; isotype: Mouse IgG1; reactivity: Human,Mouse,Rat; observed MW: 200 kDa; calculated MW: 115378 MW.
Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: NEFH — Neurofilament heavy polypeptide
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: N52; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 200 kDa; Calculated: 115378 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Scientific background (datasheet): Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).
Cellular localization (datasheet): Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Tissue details (datasheet): Muscle specific. Located in the thin filament of striated muscle.
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Alzheimer's Disease,Cell Adhesion Proteins,Cell Type Marker,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Neurodegenerative Disease,Neurology Process,Neuron Marker,Neuroscience,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
