| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;Nefh;Nfh; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone N52 is a mouse monoclonal antibody that recognizes neurofilament heavy polypeptide (gene Nefh) in human, mouse and rat samples and is validated for Western blot and IHC.
Neurofilament heavy chain (NF-H, NEFH) is one of the three subunits of neurofilaments, the intermediate filaments of neurons, together with NF-L and NF-M. Its long, heavily phosphorylated tail is abundant in mature axons and helps set axon caliber, and NF-H antibodies are common markers of neurons and axons.
| Target | Neurofilament heavy polypeptide (gene Nefh; UniProt P16884, rat) |
|---|---|
| Clone | N52 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human, Mouse, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 1–2 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.5 mL (Human, Mouse, Rat) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neurofilament heavy polypeptide in the cytoplasm and cell projection. Tissue expression noted by UniProt (rat): Expressed in the dorsal root ganglion neurons (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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