| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase Nek2; HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1; NEK2; NEK2A; NLK1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NEK2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21N32 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase Nek2 in human samples and is validated for Western blot. The predicted molecular weight is 51.8 kDa; the supplier reports observed bands at 48, 44 kDa.
NEK2 is a NIMA-related serine/threonine kinase that peaks at the centrosome in late G2. It phosphorylates linker proteins such as C-NAP1 (CEP250) and rootletin, releasing the two centrosomes so they can separate and form a bipolar spindle, and its frequent overexpression in tumors has made it a subject of cancer research.
| Target | Serine/threonine-protein kinase Nek2 (gene NEK2; UniProt P51955, human) |
|---|---|
| Clone | 21N32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.8 kDa |
| Observed MW | 48, 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human NEK2.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
