| Field | Specification |
|---|---|
| Target | |
| Alternative names | Negative elongation factor A; NELF-A; Wolf-Hirschhorn syndrome candidate 2 protein; NELFA; WHSC2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NELFA recombinant protein (Position: I38-L482). Human NELFA shares 94.6% |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets negative elongation factor A (gene NELFA) in human samples and is validated for Western blot, flow cytometry and ELISA.
NELFA is a subunit of the negative elongation factor (NELF) complex, which works with DSIF to pause RNA polymerase II shortly after transcription starts. Release of this promoter-proximal pause by the P-TEFb kinase is a key step in gene regulation, and NELF has also been linked to HIV-1 latency.
| Target | Negative elongation factor A (gene NELFA; UniProt Q9H3P2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NELFA fragment (Ile38–Leu482), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places negative elongation factor A in the nucleus. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
