Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody

SKU:BHA21007827
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Boster Bio
Boster Bio
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Overview
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Anti-RBFOX3 antibody (Rabbit, Monoclonal, clone Clone: AO-18, Rabbit IgG) recommended for WB, IHC, ICC, IF Commonly used in Neuroscience studies where these format selectors and application compatibility are required.
Target RBFOX3
Clone number Clone: AO-18
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, IHC, ICC, IF
Options selector
Catalog no. Size Conjugation
M11954 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB,IHC,ICC/IF,IF
      Application details: WB 1:500-2000
      IHC 1:50-200
      IF 1:50-200
      ICC/IF 1:50-200
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No M11954
Alternative Names RNA binding protein fox-1 homolog 3;Fox-1 homolog C;RBFOX3;
Cellular Localization Nucleus . Cytoplasm .
Clonality
  • Monoclonal
Concentration 0.5mg/ml
Gene ID 146713
Host Rabbit
Immunogen A synthesized peptide derived from human NeuN
Isotype
  • Rabbit IgG
Molecular Weight 46, 50 kDa
Product Type
  • Antibodies
  • Primary Antibodies
Reactivity
  • Human
  • Mouse
  • Rat
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Target RBFOX3
UniProt # A6NFN3

Overview

Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody is an antibody targeting RBFOX3. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AO-18; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 46, 50 kDa; calculated MW: 34 kDa.

Boster Bio Anti-NeuN RBFOX3 Rabbit Monoclonal Antibody catalog # M11954. Tested in WB, IHC, ICC/IF, IF applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

  • Target: RBFOX3 — RNA binding protein fox-1 homolog 3
  • Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AO-18; Isotype: Rabbit IgG
  • Species reactivity: Human,Mouse,Rat
  • Molecular weight guidance: Observed: 46, 50 kDa; Calculated: 34 kDa

Biological background

Protein function (datasheet): RNA-binding protein that regulates alternative splicing events. .

Cellular localization (datasheet): Nucleus . Cytoplasm .

Tissue details (datasheet): Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .

Research relevance and current trends

  • Commonly studied in contexts related to Cell Type Marker,Cell Type Markers,Domain Families,Epigenetics and Nuclear Signaling,Forkhead Box,Neuron Marker,Neuroscience,Tags & Cell Markers,Transcription,Transcription Factors.
  • Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
  • Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

  • Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
  • Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
  • Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.

Notes for experimental interpretation

  • Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
  • Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Will anti-NeuN Rabbit Monoclonal antibody M11954 work for IF with cerebellum?
According to the expression profile of cerebellum, RBFOX3 is highly expressed in cerebellum. So, it is likely that anti-NeuN Rabbit Monoclonal antibody M11954 will work for IF with cerebellum.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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