| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neurocan core protein; Chondroitin sulfate proteoglycan 3; NCAN; CSPG3, NEUR; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Neurocan, which shares 90% amino acid (aa) sequence identity with both mouse and rat Neurocan. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of NCAN (AP-2 complex subunit beta) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Neurocan/NCAN Antibody Picoband® catalog # A06700-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human Neurocan, which shares 90% amino acid (aa) sequence identity with both mouse and rat Neurocan.
- Molecular weight context: reported MW: 200 kDa; calculated MW: 104553 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
AP-2 complex subunit beta; neurocan. Neurocan core protein is a protein that in humans is encoded by the NCAN gene. Neurocan is a member of the lectican / chondroitin sulfate proteoglycan protein families and consists of neurocan core protein and chondroitin sulfate. It is thought to be involved in the modulation of cell adhesion and migration. In the human subjects, it was found that NCAN genotype was strongly associated with manic symptoms but not with depressive symptoms. In the mice, the absence of functional Neurocan resulted in a variety of manic-like behaviors, which could be normalized by administering lithium. Functional note: May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. Reported localization: Secreted . Expression/tissue context: Brain.
Research relevance and current trends
- Cell Adhesion Proteins: Researchers commonly examine how NCAN (AP-2 complex subunit beta) relates to this theme using model systems and orthogonal readouts.
- ECM Proteins: Researchers commonly examine how NCAN (AP-2 complex subunit beta) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how NCAN (AP-2 complex subunit beta) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative NCAN (AP-2 complex subunit beta) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of NCAN (AP-2 complex subunit beta) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
