| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurocan core protein; Chondroitin sulfate proteoglycan 3; NCAN; CSPG3; NEUR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Neurocan |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25N29 is a rabbit monoclonal antibody that recognizes neurocan core protein (gene NCAN) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 143.1 kDa; the supplier reports observed bands at 130 kDa, 270 kDa.
Neurocan (NCAN) is a chondroitin sulfate proteoglycan of the lectican family, expressed mainly in the developing brain. It binds hyaluronic acid and neural cell adhesion molecules and helps form the brain extracellular matrix, influencing neuronal adhesion, neurite outgrowth and perineuronal nets.
| Target | Neurocan core protein (gene NCAN; UniProt O14594, human) |
|---|---|
| Clone | 25N29 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 143.1 kDa |
| Observed MW | 130 kDa, 270 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Fetal brain cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human neurocan.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes neurocan core protein as a secreted protein. Tissue expression noted by UniProt (human): Detected in cerebrospinal fluid (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
