| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein phosphatase 1 regulatory subunit 14A;17 kDa PKC-potentiated inhibitory protein of PP1;Protein kinase C-potentiated inhibitor protein of 17 kDa;CPI-17;PPP1R14A;CPI17, PPP1INL; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NRGN recombinant protein (Position: K11-K54). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Neurogranin/NRGN Antibody Picoband® is an antibody for NRGN detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IHC-P, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: NRGN (Protein phosphatase 1 regulatory subunit 14A); UniProt: Q92686
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 11 kDa, calculated 16693 MW
- Applications: WB, IHC, IHC-P, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Neurogranin/NRGN Antibody Picoband® catalog # A05781-3.
Biological background
Biological context: Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Expression and localization notes: cellular localization: Cytoplasm ., tissue context: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. ..
Common research applications
- Western blotting (WB): Compare NRGN levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of NRGN in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify NRGN-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Neurogranin is a calmodulin-binding protein expressed primarily in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Cytoplasm .
- Tissue details: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .
- Research category: Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
