| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuropilin-1; Vascular endothelial cell growth factor 165 receptor; CD antigen CD304; NRP1; NRP; VEGF165R |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 8G6G7 is a mouse monoclonal antibody that recognizes neuropilin-1 (gene NRP1) in human and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 103.1 kDa, and the supplier reports an observed band at about 120 kDa.
Neuropilin-1 (NRP1) is a transmembrane co-receptor for class 3 semaphorins and for VEGF-A isoforms such as VEGF165. It shapes axon guidance, blood vessel formation and heart development, and it is expressed on regulatory T cells. NRP1 also binds C-end rule peptide motifs and is studied in angiogenesis, neural development, immunity and cancer.
| Target | Neuropilin-1 (gene NRP1; UniProt O14786, human) |
|---|---|
| Clone | 8G6G7 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 103.1 kDa |
| Observed MW | 120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human placenta tissue, human U-87MG cells, human PC-3 cells, human MCF-7 cells, rat stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human Neuropilin 1 fragment (Lys504–Thr827), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95% and 94%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. Tissue expression noted by UniProt (human): The expression of isoforms 1 and 2 does not seem to overlap. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
